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As with correlation analyses between the different milk composition QTLs, P values for the MGST1 expression and milk fat and protein percentage phenotypes were highly correlated (=0.73; Fig. 5E), where the g.93945738 'C' allele corresponded to increased MGST1 expression and increased milk fat percentage. These data suggested that variants affecting expression of MGST1 were responsible for the differences in milk composition demonstrated at this locus, confirming this as the causative gene for these effects.

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