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AbstractThe objective of this study was to examine five different sources of RNA, namely mammary gland tissue (MGT), milk somatic cells (SC), laser microdissected mammary epithelial cells (LCMEC), milk fat globules (MFG) and antibody captured milk mammary epithelial cells (mMEC) to analyze the bovine mammary gland transcriptome using RNA Sequencing. Our results provide a comparison between different sampling methods (invasive and non invasive) to define the transcriptome of mammary gland tissue and milk cells. This information will be of value to investigators in choosing the most appropriate sampling method for different research applications to study specific physiological states during lactation.
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