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Critically, the MGST1 eQTL located to the same position as the milk composition QTLs, and shared the same lead SNP (g.93945738C>T) with the milk fat percentage analysis (Fig. 5E). Manual inspection of sequence alignments in animals of opposing eQTL genotype revealed uniform transcript structures with splice sites conforming to the Ensembl reference annotation, suggesting a transcriptional (versus splicing) based mechanism to the eQTL.

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